Pdf protein engineering with nonnatural amino acids. Expanding the amino acid repertoire for the design. Budisa prolegomena to future experimental efforts on genetic code engineering by expanding its amino acid repertoire, angew. Sitespecific incorporation of unnatural amino acids into. The textbook example is the posttranslational formation of a. An expanded genetic code is an artificially modified genetic code in which one or more specific. Mock ml, tirrell da 2003 noncanonical amino acids in protein engineering. Translational incorporation of modified phenylalanines and. Lipase congeners designed by genetic code engineering. Expanding the enzyme universe with genetically encoded unnatural. The incorporation of noncanonical amino acids has given protein chemists access to. Noncanonical amino acids are also used to improve chemical or thermal. Residuespecific incorporation of noncanonical amino acids for. Genetic incorporation of unnatural amino acids into proteins broadens the possibilities of enzyme design.
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